Journal: JHEP Reports

Article Title: Hepatic immune environment differences among common mouse strains in models of MASH and liver cancer

doi: 10.1016/j.jhepr.2025.101380

Figure Lengend Snippet: Changes of liver immune subsets under MASH in BALB/c, C57BL/6, and FVB/N mice fed with MCD diet. Female BALB/c, C57BL/6, and FVB/N mice were fed with MCD diet ( vs. regular diet) to induce MASH. (A) The development of MASH was confirmed by H&E staining. Liver immune cells from MASH mice or control mice were prepared and immune subsets were measured by flow cytometry analysis. The comparison between MCD (green) and control (gray) was performed in each liver immune subsets of the three mouse strains (B–G). The overall changes of various liver immune subsets from three mouse strains are shown (H). The size of circle represents the log 10 transformed fold changes of each immune subset. The color gradient represents the relative frequences of each immune subset. The distribution of fold changes of each type of immune cell is also shown; n = 4 per group, two-way ANOVA with Bonferroni correction, ∗ p <0.05. MASH, metabolic dysfunction-associated steatohepatitis; MCD diet, methionine- and choline-deficient diet; Tregs, regulatory T cells.

Article Snippet: MASH was induced in mice by feeding a methionine- and choline-deficient (MCD) diet (Research diets inc., New Brunswick, NJ, USA Ref. A02082002BR) for a period of 3 weeks, or a Western diet (Envigo, Indianapolis, IN, USA, Ref. TD.120528) with high sugar solution (23.1 g/L d-fructose and 18.9 g/L d-glucose) with weekly intraperitoneal injections of carbon tetrachloride (CCl 4 ) (Sigma, St. Louis, MO, USA, Cat# 289116) at a dose of 0.32 mg/g of body weight for 12 weeks as previously reported.

Techniques: Staining, Control, Flow Cytometry, Comparison, Transformation Assay

Journal: JHEP Reports

Article Title: Hepatic immune environment differences among common mouse strains in models of MASH and liver cancer

doi: 10.1016/j.jhepr.2025.101380

Figure Lengend Snippet: Cross-species comparison of liver immune changes by MASH between mice and humans. The published human scRNA-seq dataset GSE159977 of CD45+ cells from either MASH or healthy livers were processed using Seurat (5.1.0). (A) shows the UMAP split based on healthy or MASH. (B) shows the dot plot of marker genes for each annotated cell clusters. (C) Liver CD45 + cell compositions were measured in naïve BALB/c, C57BL/6, and FVB/N strains by flow cytometry as described in Fig. 1 A. CD45 + cell composition of healthy human liver or human HCC adjacent liver tissues were calculated based on the scRNA-seq datasets of GSE159977 or phs003279.v1.p1, respectively. (D,E) CD45 + or CD4 + T cell compositions of MASH or healthy human livers were calculated from GSE159977 . (F) In total liver CD45 + cells, the frequencies of shared major liver immune subsets between mice and human, including CD8+ T cell, CD4+ T cell, B cells, γδT cells and NK cells, were calculated in MASH or healthy human livers ( GSE159977 ) and MASH or control mice of three strains under either MCD diet or Western + CCl 4 diet MASH model. CCl 4 , carbon tetrachloride; HCC, hepatocellular carcinoma; MASH, metabolic dysfunction-associated steatohepatitis; MAIT cells, mucosal-associated invariant T cells; MCD diet, methionine- and choline-deficient diet; NK, natural killer; UMAP, Uniform Manifold Approximation and Projection.

Article Snippet: MASH was induced in mice by feeding a methionine- and choline-deficient (MCD) diet (Research diets inc., New Brunswick, NJ, USA Ref. A02082002BR) for a period of 3 weeks, or a Western diet (Envigo, Indianapolis, IN, USA, Ref. TD.120528) with high sugar solution (23.1 g/L d-fructose and 18.9 g/L d-glucose) with weekly intraperitoneal injections of carbon tetrachloride (CCl 4 ) (Sigma, St. Louis, MO, USA, Cat# 289116) at a dose of 0.32 mg/g of body weight for 12 weeks as previously reported.

Techniques: Comparison, Marker, Flow Cytometry, Control, Western Blot